Choose an “Unknown Culture” and do the following: Write down the number of your unknown on a fresh page in your notebook.

Biology

Choose an “Unknown Culture” and do the following:

1. Write down the number of your unknown on a fresh page in your notebook.

2. Prepare a smear and do a Gram Stain

a. Write the results of the Gram stain under your unknown number in your notebook and take a picture.

b. Show the instructor your Gram Stain at 1000x magnification.

3. Inoculate the following plates for isolation:

a. Blood Agar Plate (BA)

b. Mac Conkey Agar Plate (MAC)

c. PEA Agar Plate

d. Nutrient Agar Plate

e. Mannitol Salt Agar Plate (MS)