Calculate the change in absorbance between Time 0 and Time 50 from the Benedicts test and place the results in Table 4.

Biology Report

The data you will need to answer the following questions are listed in Table 1.

Table 1: Color, estimated pH and absorption every 10 min from the bicarbonate solution.

Time (min) W Syringe G Syringe
Color Estimated pH Absorption Å Color Estimated pH Absorption Å
0

 

Red/Purple 8.8 0.779 Red/Purple 8.8 0.761
10

 

Red/Purple 8.8 0.734 Red 8.6 0.624
20

 

Red/Purple 8.8 0.751 Red 8.6 0.603
30

 

Red/Purple 8.8 0.753 Dark 0range 8.4 0.583
40

 

Red/Purple 8.8 0.753 Dark orange 8.4 0.504
50

 

Red/Purple 8.8 0.751 Orange 8.2 0.433

 

Time (min) Mg Syringe
Color Estimated pH Absorption Å

 

0

 

Red/Purple 8.8 0.729
10

 

Red 8.6 0.607
20

 

Dark 0range 8.4 0.529
30

 

Orange 8.2 0.407
40

 

Orange/Yellow 8.2 0.334
50

 

Orange/Yellow 7.8 0.308

 

 

 

 

2) (2 pnts) Calculate the change in absorption using the formula ABS0 – ABST (Absorption at time 0 min – the Absorption at time T min) and place the values in Table 2.

 

Table 2: Change in absorption over time for the bicarbonate solutions.

Time (Min) W Syringe G Syringe Mg Syringe
0

 

0 0 0
10

 

     
20

 

     
30

 

     
40

 

     
50

 

     

 

 

3) (4pnts) Graph (use scatter plot) the change in absorption over time for all 3 treatments (W, G, Mg) and paste the graph below. Be sure to label the axes.

 

 

 

4) (3pnts) Which reaction produced CO2 the fastest? Use the rate of color change and the change in absorption to answer the question.

 

 

 

 

Data collected from conducting the Benedicts test are listed in Table 3.

 

Table 3: Results of Benedicts test

Treatment Time 0  Absorption Å Time 0 Color Time 50 Absorption Å Time 50 Color
W

 

0.021 Blue 0.022 Blue
G

 

0.593 Orange/Red 0.379 Yellow/Orange
Mg

 

0.589 Orange/Red 0.182 Green/yellow

 

 

 

 

 

 

 

 

 

6) (1pnts) Calculate the change in absorbance between Time 0 and Time 50 from the Benedicts test and place the results in Table 4.

 

Table 4: Change in absorbance in the Benedicts test

Treatment Time 0 – Time 50 Å

 

W

 

 
G

 

 
Mg

 

 

 

 

7) (4pnts) Which reaction showed the loss of most of the glucose? Justify your answer with the data you collected.

 

 

 

8) (4pnts) Based on the both data sets (bicarbonate and Benedicts) what affect did Mg have on the rate of the reaction? Justify your answer with both data sets. (Note: if you know there was an issue with some of your data you can explain what you expected to happen and why your results do not match the expectation).